If you've been working 3 weeks straight without a day off (albeit you've only worked half days on the weekends) and then you sort-of mess up a protocol (by losing some sample) do you:
(a) Start prepping a new batch of samples while continuing with your diminished-sample prep because, hey! maybe diminished-sample prep will be enough (at the very least it may still tell you what you need to know). But know that you'll lose your Saturday (with your significant other) off.
(b) Just toss everything as (now) diminished-sample prep was a trial for your new preparative method and start over. And lose your Saturday (with your significant other) off.
(c) Just like (a) but decide that you'll take Saturday off anyhow and just come in Sunday.
(d) Just like (a) but decide that you fail at life.
14 hours ago